Figure 9.

Depletion of PARP-1 alone significantly increases the frequency of micronuclei and blocks the ST-dependent autophagy. (a and b) After transfection with p62 siRNA for 48 h, 786-O cells were incubated with ST for 3 h, lysed, and the lysates were precipitated using the antibody against γ-H2AX or Rad51. The immunoprecipitates were resolved by electrophoresis and probed by immunoblotting with the indicated antibodies. (c–f) 786-O cells were transfected with siRNA target PARP-1 for 48 h. (c) Following treatment with ST with or without CQ for 12 h, images were obtained using fluorescence microscopy, and the histogram graph data representing the mean±S.D. were shown in (d), **P<0.01 versus control. (e and f) Cell lysates were prepared and immunoblotting was performed with the indicated antibodies for 3 h. The data represent three independent experiments. **P<0.01, and ns was short for insignificance