Figure 1.

TTN exhibited anti-inflammatory effects in lipopolysaccharide (LPS)-stimulated RAW264.7 cells. (a) HPLC chromatogram of TTN. (a) DTN (5.34%); (b) TNI (19.81%); (c) CTN (22.12%); (d) TNA (45.12%). (b) RAW264.7 cells were pretreated with TNI, TNA, CTN, DTN, or TTN for 1 h before LPS (1 μg/ml) stimulation for another 24 h. Nitrite production was determined by Griess assay. (c and d) RAW264.7 cells were pretreated with TNI (4 μg/ml), TNA (4 μg/ml), CTN (4 μg/ml), DTN (4 μg/ml), or TTN (4 μg/ml) for 1 h before LPS (1 μg/ml) stimulation for another 6 h. NO was determined by flow cytometry with DAF-FM (1 μM). The values were expressed as means±S.D. *P<0.05 and **P<0.01 versus LPS alone group, n=6