Figure 8.

TTN suppressed the release of inflammatory cytokines and renal injury and improved kidney injury in lipopolysaccharide (LPS)-stimulated AKI mice. BALB/c mice were pretreated with TTN (20, 40, and 80 mg/kg, intraperitoneal (i.p.)) for 2 h before LPS (10 mg/kg, i.p.) injection. After 12 h, blood samples were collected via the retro-orbital route under anesthesia. Tumor necrosis factor-α (a), interleukin (IL)-6 (b), and IL-1β (c) were determined by ELISA kits, and blood urea nitrogen (d) and creatinine (e) were examined by Roche Modular P800. H&E staining (magnification, × 400). (f) Control group; (g) LPS (10 mg/kg, i.p.) group; (h) TTN (20 mg/kg, i.p.) group; (i) TTN (40 mg/kg, i.p.) group; (j) TTN (80 mg/kg, i.p.) group; (k) DEX (5 mg/kg, i.p.). DEX, positive control (n=10). The values were expressed as means±S.D. *P<0.05, **P<0.01, and ***P<0.001 versus LPS alone group