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. 2017 Aug 10;8(8):e2989. doi: 10.1038/cddis.2017.391

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Copyright © 2017 The Author(s)

Cell Death and Disease is an open-access journal published by Nature Publishing Group. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Dioscin exhibited cytotoxicity in PC3 cells, and disrupted mammospheres formation. (a) Effects of dioscin on the viabilities of PC3 cells and PC3 cell-derived mammospheres. (b) Effects of different concentrations of dioscin (1.4, 2.8 and 5.6 μM) for 24 h on the morphology and structure of PC3 cells (bright-field image). (c) Effects of different concentrations of dioscin (2.5, 5.0 and 10.0 μM) for 24 h on the morphology and structure of PC3 cell-derived mammospheres (bright-field image). (d) Mammosphere revealing cells staining positive for Aldefluor (green), counterstained with Hoechst 33342 (blue). Data are presented as the mean±S.D. (n=6)