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. 2017 Aug 10;8(8):e2989. doi: 10.1038/cddis.2017.391

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Copyright © 2017 The Author(s)

Cell Death and Disease is an open-access journal published by Nature Publishing Group. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Dioscin-induced apoptosis in PC3 cells and mammospheres. (a) Effects of dioscin (1.4, 2.8 and 5.6 μM) for 24 h on apoptosis of PC3 cells using flow cytometry analysis. (b) Effects of dioscin (2.5, 5.0 and 10.0 μM) for 24 h on apoptosis of PC3 cell-derived mammospheres using flow cytometry analysis. (c) Effects of dioscin (1.4, 2.8 and 5.6 μM) for 24 h on cleaved caspase-3, cleaved PARP, Bax and Bcl-2 expression levels in PC3 cells. (d) Effects of dioscin (2.5, 5.0 and 10.0 μM) for 24 h on cleaved caspase-3, cleaved PARP, Bax and Bcl-2 expression levels in PC3 cell-derived mammospheres. Data are presented as the mean±S.D. (n=5).*P<0.05 and **P<0.01 versus Control group