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. 2017 Aug 31;8(8):e3023. doi: 10.1038/cddis.2017.404

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Copyright © 2017 The Author(s)

Cell Death and Disease is an open-access journal published by Nature Publishing Group. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Extracellular FGF1 and etoposide increase endogenous fgf1 expression in SH-SY5Y cells, in contrast to N2a cells. SH-SY5Y and N2a cells were treated or not with rFGF1 for 72 h (a–c) or etoposide for 16 h (b–d). The levels of all fgf1 mRNAs (a,b) or of the alternative 1B fgf1 mRNA (c,d) were analyzed by RT-PCR. The 18S rRNA levels were used as a control for quantifications. The graphs represent the mean ±S.E.M. of three independent experiments. Student’s t-tests were performed (n=3; n.s.: P>0.05; *P⩽0.05; **P⩽0.01; ***P⩽0.001)