Table 1. Alterations of mRNA and protein expression in the BRAFi-resistant cell lines compared with parental A375 cells.

MAPK qPCR pathway array (log2)				Proteomics (log2)
Symbol	A375PR1	A375VR3	A375VR4	Symbol	A375PR1	A375VR3	A375VR4
CCNA2	−0.41	1.02	1.11	CCNA2	0.29	0.10	−0.07
CCND1	1.00	3.70	4.05	CCND1	−0.30	1.02	1.28
CDC42	0.01	1.51	1.31	CDC42	0.14	−0.16	0.14
CDK2	1.34	2.21	1.59	CDK2	−0.25	0.03	−0.32
CDK6	−1.27	0.47	0.25	CDK6	−1.26	−0.27	−0.73
CDKN1A	−0.77	0.51	1.03	CDKN1A	−0.13	0.85	1.53
CDKN2C	−0.25	1.46	1.28	CDKN2C	−0.36	0.66	0.68
CREBBP	−0.33	2.92	2.84	CREBBP	0.09	0.18	0.19
EGFR	0.69	1.14	2.00	EGFR	0.63	0.56	1.24
ETS1	−2.45	2.17	2.48	ETS1	−1.17	−0.15	−0.34
GRB2	−0.16	1.08	0.96	GRB2	−1.00	0.05	−0.22
HSPA5	0.86	1.10	1.02	HSPA5	0.38	0.18	0.31
HSPB1	1.31	−1.07	−3.34	HSPB1	0.08	0.20	−0.84
JUN	−1.48	1.73	1.39	JUN	0.29	0.14	0.28
KRAS	−0.02	1.86	1.73	KRAS	0.15	0.01	−0.16
MAP2K1	−0.10	1.87	1.98	MAP2K1	−0.56	0.52	0.55
LAMTOR3	−0.32	−3.22	−3.77	LAMTOR3	0.40	0.22	0.16
MAP2K2	−1.40	1.23	1.03	MAP2K2	−1.22	−0.53	−0.51
MAP2K3	0.10	1.47	0.99	MAP2K3	−0.51	−0.39	−0.75
MAP2K4	−0.89	0.82	1.22	MAP2K4	−1.03	0.38	0.77
MAP2K7	−0.86	3.16	3.56	MAP2K7	−1.33	0.08	0.003
MAPK3	0.15	1.61	1.37	MAPK3	−0.76	−0.31	−0.58
MAX	−0.52	1.02	0.79	MAX	0.09	−0.07	−0.14
PAK1	−0.003	2.13	2.27	PAK1	−0.81	−0.34	−0.50
RAF1	−0.71	1.34	1.86	RAF1	−0.45	−0.13	−0.31
SMAD4	−0.05	1.07	0.79	SMAD4	−0.48	−0.17	−0.30

MAPK pathway array and corresponding protein data from proteomics indicate gene/protein expression alterations in the BRAFi-resistant cells. A negative log2 value is indicative of downregulation and a positive log2 value shows upregulation of mRNA/protein expression