Table 2. Anlysis of selected mRNA and protein expression alterations in the BRAFi-resistant cell lines compared with A375 parental cells.
|
RNA |
Protein |
||||||
|---|---|---|---|---|---|---|---|
| Genes | A375PR1 | A375RV3 | A375RV4 | A375PR1 | A375VR3 | A375VR4 | Protein |
| ALCAM | ND | ND | 1.21 | −0.15 | 0.83 | 1.23 | CD166 |
| ANPEP | ND | ND | 3.8 | 1.88 | 1.96 | 2.73 | CD13 |
| CCND1 | 1 | 3.7 | 4.05 | −0.3 | 1.02 | 1.28 | CCND1 |
| EFNA1 | ND | ND | −0.45 | −0.42 | −1.17 | −1.27 | EFNA1* |
| EGFR | 0.69 | 1.14 | 2 | 0.63 | 0.56 | 1.24 | EGFR |
| EPHA2 | 1.32 | 2.77 | 3.29 | 0.46 | 1.86 | 2.3 | EPHA2 |
| FLI1 | ND | ND | 2.7 | −1.16 | 0.79 | 1.61 | FLI1* |
| MET | -0.07 | 0.5 | 0.88 | 1.08 | 0.89 | 1.28 | MET |
| MITF | ND | ND | −2.43 | −0.42 | −1 | −1.34 | MITF |
| PTEN | ND | ND | ND | −1.19 | −0.5 | −0.48 | PTEN |
| SOX10 | ND | ND | −2.03 | −0.36 | −0.81 | −1.13 | SOX10 |
A negative log2 value is indicative of downregulation and a positive log2 value shows upregulation of mRNA or protein expression*Measurement from immunoblotting