Figure 5.

Inhibition of hexokinase rescues CQ-dependent cell death. (a) 4T1 cells were treated with full-nutrient DMEM or serum-free DMEM, in the presence of 2DG (5 mM) ±CQ (25 μM) as indicated, for 24 h. After this incubation, cell clonogenic viability was measured (n=3 experiments ±S.E.M.). ***P<0.001 by one-way ANOVA. (b) Cells were treated with serum-free DMEM, or serum-free and glucose-free DMEM+CQ (25 μM) for 24 h, followed by cell viability measurements. Where indicated, incubations included methyl pyruvate (10 mM) or galactose (Gal, 5 mM). (c) Cells were treated the following nutrient conditions: (1) full-nutrient DMEM; (2) serum-free DMEM; (3) serum- and glucose-free DMEM; or (4) serum-, glucose- and glutamine-free DMEM, ±CQ (25 μM), as indicated for 24 h, followed by clonogenic viability measurements