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. 2017 Aug 24;8(8):e3011. doi: 10.1038/cddis.2017.421

Figure 5.

Figure 5

Reciprocal repression between lncRNA XIST and miR-200b-3p. (a) Schematic representation of the predicted target site for miR-200b-3p in lncRNA XIST. (b) Knockdown of lncRNA XIST increased miR-200b-3p expression in HCT116 cells; ectopic miR-200b-3p expression decreased lncRNA XIST expression, and inhibition of miR-200b-3p increased lncRNA XIST expression (*P<0.05). (c) Luciferase reporter assay in human embryonic kidney (HEK) 293T cells, co-transfected with the reporter plasmid (or the corresponding mutant reporter) and the indicated miRNAs. miR-200b-3p significantly decreased the luciferase activity in XIST-wt but not in XIST-mt (*P<0.05). (d) The expression of lncRNA XIST was inversely correlated with the expression level of miR-200b-3p in CRC tissues (r=−0.73, *P=0.013). Error bars represent the mean±S.D. values