Motility (mean ± SD) of thawed sperm with four cryoprotectants (at 10% concentration) at different cooling rates from 5 to -80 °C in Trial 1(top panel), and Trial 2 (bottom panel): methanol, white; 2-methoxyethanol (ME), light gray; dimethyl sulfoxide (Me2SO), dark gray (Trial 1 only), and N, N-dimethyl formamide (DMF), black. The motility of fresh sperm (before addition of cryoprotectants) was 85 ± 0 % in Trial 1, and 83 ± 3% in Trial 2.