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. Author manuscript; available in PMC: 2018 Jan 15.
Published in final edited form as: Cancer Res. 2016 Nov 4;77(2):448–458. doi: 10.1158/0008-5472.CAN-16-2350

Figure 5. Tempol treatment suppresses 4-OHE2-induced DNA damage in vivo and delays the breast tumor onset in parous BBP mice.

Figure 5

(A) Tempol decreases the DNA adducts in mammary gland of parous BBP mice. The levels of 4-OHE2-1-N3Ade and 4-OHE2-1-N7Gua in mammary gland of the virgin and parous BBP mice (25 weeks) treated with or without tempol were examined by mass spectrometry. *, p < 0.01. (B, C) Tempol treatment suppresses AP sites (B) and DSBs (C) in the mammary glands of parous BBP mice (40 weeks). Immunostaining of γH2AX indicates DSBs. Scale bar: 50 µm. (D) Kaplan-Meier analysis of the mammary tumor free fraction of parous BBP mice treated with mock or tempol. The median tumor free fractions are 302 and 423 days, respectively.