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. 2017 Oct;363(1):12–19. doi: 10.1124/jpet.117.243246

Fig. 2.

Fig. 2.

Reversed-phase LC selected ion chromatograms of ponatinib oxidation and GSH adduct mediated by CYP1A1. Ponatinib (50 µM) was incubated with CYP1A1 in the presence of an NADPH regeneration system as described in Materials and Methods. (A) Formation of P-OH (m/z 549.22) (top) in the absence of GSH and mGstp1, and (middle) in the presence of 5 mM GSH and 10 µg/ml mGstp1; (bottom) formation of P-GSH (m/z, 2+, 419.65) in the presence of 5 mM GSH and 10 µg/ml mGstp1. (B) Formation of P-2OH (m/z 565.22) (top) in the absence of GSH and mGstp1 and (middle) in the presence of 5 mM GSH and 10 µg/ml mGstp1; (bottom) Formation of P-OH-GSH (m/z, 2+, 427.65) in the presence of 5 mM GSH and 10 µg/ml mGstp1.