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. 2017 Sep 12;14:175. doi: 10.1186/s12985-017-0846-x

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — The algorithm used in this study. A depicts the 5′-UTR assay. B and C show the two VP1 assays. While B has only one stage of PCR, C has two consecutive stages (snPCR) of PCR