Fig. 2.

Ethanol inhibition in high-gravity SSCF with S. cerevisiae KE6-12.A. Shake flask SSCF of 20% (w/w) WIS of steam-pretreated wheat straw (material M1) with 2-h pre-hydrolysis, 10 FPU (g WIS)⁻¹ enzyme dosage, at 35 °C. The addition of approximately 75 or 50 g L⁻¹ ethanol severely inhibited ethanol production. Results shown are average values of duplicate experiments, and the relative difference between duplicate experiments was lower than 5%