Fig. 3.

In vitro promotion of aggressiveness in astrocytoma by the upregulation of NUSAP1. a Gene set enrichment analysis (GSEA) showed a significant correlation between NUSAP1 mRNA expression and the proliferation-associated gene signature. b Western blotting was used to examine the protein expression of NUSAP1 in SW 1008 and U-87 MG cells. α-Tubulin was used as a loading control. c In the MTT assay, overexpression of NUSAP1 significantly increased the growth rate of the indicated cells, while downregulation of NUSAP1 decreased the growth rate of the indicated cells. Error bars represent the mean ± SD values of three independent experiments. d Representative images (left panel) and quantification (right panel) of cells in the colony formation assay. Overexpression of NUSAP1 increased, while downregulation of NUSAP1 decreased, the colony-forming ability of the indicated cells. Error bars represent the mean ± SD values of three independent experiments (*P < 0.05). e Representative images (left panel) and quantification (right panel) of the indicated invaded cells analyzed by the Transwell matrix penetration assay. Error bars represent the mean ± SD values of three independent experiments (*P < 0.05). f The wound-healing assay was conducted with the indicated cells, and images were taken at 0 and 24 h. Overexpression of NUSAP1 increased, while downregulation of NUSAP1 decreased, the migration ability of the indicated cells