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Reverse transcriptase PCR of mRNA extracted from microbiopsies (MBs) for detection of skin cells. The first strand cDNA was prepared from total mRNA yields of: Lane 1, MB type 2 (MB2); Lanes 2,3, MB type 1 (MB1); Lane 4, negative control without (w/o) reverse transcriptase (RT); Lane 5, negative control without mRNA; MW, DNA size markers. (A) Keratin (skin-specific) cDNA, (107 bp). (B) β–Actin (housekeeping gene) cDNA (800 bp).
