Figure 3.

L253X/X has measurable gene expression differences relative to Crx^−/−. (A) Expression at P10 of the indicated photoreceptor genes was measured by qRT-PCR and compared between L253X/X and Crx^−/− at P10. The gray dashed lines mark 2-fold differences (±1 log₂ relative expression) between the two genotypes (mean ± SEM, n ≥ 3; * P < 0.05, ** P < 0.01, **** P < 0.0001; unpaired t-test with Welch's Correction). (B–D) RHO immunostaining of retinal sections of the indicated mice at 1 mo revealed less RHO expression in L253X/X retinas than Crx^−/− (signal in black, scale bar: 25 μm). (E–G) DAPI counterstaining of the above sections (signal in black).