Figure 5.

L253X/+ retinas appear morphologically similar to WT up to 3 mo. H&E-stained retinal sections from L253X/+, L253X/X, and WT mice at the indicated ages: 1 mo (A–C); 2 mo (E–G); and 3 mo (I–K) were imaged at ×40 magnification (scale bar: 25 μm). Images were taken approximately 500 μm from the ONH, where differences in layer thickness are less significant. (D, H, L) ONL thickness was quantified by morphometry at five set distances (in microns) on either side of the ONH (mean ± SEM, n ≥ 3; * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001; 2-way ANOVA with Tukey's multiple comparisons test).