Figure 3.

Effect of dimerizer treatment on the CSF-1R⁺ cell population in MAFIA mice following HSV-1 infection. (A) Summary of use of MAFIA mouse model. On the day after the last AP20187 dimerizer/VEH IP injection, mice were infected with 10³ PFU HSV-1/cornea and tissues harvested for viral titers or flow cytometry analyses at 6 days PI. (B, C) Viral content in the cornea and TG tissues of MAFIA mice treated with VEH or AP20187 and subsequently infected (at 6 days PI). Bars show mean ± SEM of viral contents in corneas (B) and TG (C) at 6 days PI by plaque assay (n = 5–6 per group from two independent experiments by unpaired T-test comparison). (D) Representative flow cytometry plots compare CD45⁺CD11b⁺GFP⁺ cell populations between VEH and AP20187-treated mice subsequently infected with HSV-1 (at 6 days PI). AP20187-treated mice show a reduction in CD45⁺CD11b⁺GFP⁺ cells infiltrating the infected cornea compared to the VEH-treated counterparts. (E) Bars summarize the mean cell count ± SEM (n = 5 per group, from two independent experiments), *P < 0.05 by unpaired T-test comparison.