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. Author manuscript; available in PMC: 2017 Sep 13.
Published in final edited form as: Methods Mol Biol. 2014;1162:189–207. doi: 10.1007/978-1-4939-0777-9_16
Temperature (ºC) Time
1 cycle Holding 95 2 minutes
25 cycles Denaturation 95 30 seconds
Annealing 54 30 seconds
Extension 73 3 minutes
1 cycle Final extension 73 11 minutes