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. Author manuscript; available in PMC: 2017 Sep 13.
Published in final edited form as: Methods Mol Biol. 2014;1162:189–207. doi: 10.1007/978-1-4939-0777-9_16
Reagent Volume
DNA (Up to 1 µg) 1 µl
10x Buffer 4 2 µl
100x BSA 0.2 µl
Restriction enzyme (NheI or MluI) 0.5 µl (2-10 units)
Nuclease-free water To a final volume of 20 µl