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High-yield synthesis of α-Gal pentasaccharyl ceramide Galα3nLc4βCer (1) by enzymatic extension of lactosyl sphingosine (LacβSph, 5) using sequential one-pot multienzyme (OPME) reactions with C18-cartridge purification for the formation of α-Gal pentasaccharyl sphingosine Galα3nLc4βSph (2) followed by a simple acylation reaction.
