Table 2.

Stability of Batch Compounded Controlled Substances.

Conditions/Results	Fentanyl	Fentanyl	Fentanyl	Hydro-morphone	Ketamine	Midazolam	Midazolam	Morphine	Morphine	Pento-barbital
concentration	10 mcg/mL in NS	10 mcg/mL in D5W	50 mcg/mL	100 mcg/mL in NS	10 mg/mL	5 mg/mL	0.4 mg/mL in D5W	1 mg/mL in NS	1 mg/mL in D5W	50 mg/mL
mobile phase(buffer: ACN)	75:25	75:25	65:35	65:35	85:15	70:30	70:30	97.5:2.5	97.5:2.5	65:35
detection wavelength (nm)	210	210	210	254	210	254	254	210	210	210
injection volume (µL)	12.5	12.5	5	5	5	5	5	5	5	5
retention time (min)	5.46	5.46	1.89	2.62	3.55	2.58	2.58	3.88	3.88	4.17
Degradants a (min)	0.9 (a,b)	0.9 (a,b)	0.8 (a,b)	1.3 (a,b)	1.9 (a), 2.3 (a)	1.0 (b)	1.0 (b)	3.1 (a, b, p)	3.1 (a, b, p)	ppt (a)
initial (concentration)b	10.6 ± 0.1 mcg/mL	10.0 ± 0.1 mcg/mL	48.3 ± 0.4 mcg/mL	98.6 ± 0.3 mcg/mL	10.1 ± 0.02 mg/mL	5.06 ± 0.06 mg/mL	0.397 ± 0.001 mg/mL	1.05 ± 0.004 mg/mL	1.02 ± 0.01 mg/mL	48.8 ± 0.8 mg/mL
week 7–8 % remaining b	104.0 ± 1.9 (day 50)	101.7 ± 3.9 (day 50)	102.7 ± 3.2 (day 50)	94.2 ± 1.6 (day 50)	102.2 ± 0.4 (day 50)	97.2 ± 1.9 (day 51)	98.5 ± 0.5 (day 51)	100.9 ± 0.2 (day 50)	100.8 ± 0.6 (day 50)	99.6 ± 2.0 (day 50)
day 100 % remaining b	101.7 ± 3.0	104.0 ± 3.3	98.3 ± 4.4	90.3 ± 0.7	100.5 ± 1.5	96.5 ± 2.6	96.4 ± 1.7	101.1 ± 0.3	100.9 ± 1.0	102.2 ± 2.2
Linearity	0.9992	0.9992	0.9999	0.9999	> 0.9999	0.9996	0.9996	0.9998	0.9998	0.9994
precision	0.33	0.33	0.87	0.17	0.29	0.36	0.50	0.10	0.10	0.97
intraday coefficient of variation	0.45	0.45	1.35	1.07	0.29	0.90	0.90	0.28	0.28	1.35
interday coefficient of variation	1.74	1.74	2.82	1.11	1.48	1.05	1.05	1.63	1.63	1.52

Notes: ^a a = acid, b = base, p = peroxide; ^b mean ± SD; Triplicate determinations of four samples.