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. 2017 Sep 13;12(9):e0183880. doi: 10.1371/journal.pone.0183880

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© 2017 Mao et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 1 — A. CD14+ PBMC obtained from HCC patients were stained and analyzed by flow cytometry. B. CD14+ monocytes were divided into two distinct subsets, CD14+CD16+ and CD14+CD16- cells. C. TEMs were examined by using TIE2-PE orIgG1-k-PEisotypic antibody. D.The percentage of TEMs in peripheral blood CD14+CD16+ monocytes for this sample was 16.14% (the percentage of TEMs in CD14+CD16+ monocytes measured by TIE2-PE staining minus that measured by IgG1-k-PE staining, 24.2% - 8.06% = 16.14%).