Fig 4. Length and RCV distribution of short annotated genes, novel genes with annotated homologs, and novel genes without annotated homologs.

(A) The ORF length in AA was binned into eight categories and the number of ORFs for each gene group belonging to every category was determined. On average, the annotated genes are longer than the novel genes. The novel genes without annotated homologs have the shortest length. (B) The translatability expressed by the ribosomal coverage value (RCV) when growing in LB. The RCV was binned into ten groups. All three gene categories show a similar RCV distribution.