Complete VP1 gene sequences (927 nt, corresponding to nucleotide positions 2,389–3,315 of the Fermon prototype strain of EV-D68) were analyzed. The phylogenetic tree was constructed and evaluated with 1,000 bootstrap pseudoreplicates using MEGA 7.0 software. Based on the Akaike information criterion with correction for finite sample sizes, a general time reversible (GTR) plus gamma distributed with invariant sites (G+I) model was used. Numbers at nodes, which indicate bootstrap support values (>85%), are shown. Sequences in GenBank were also included in the analysis. Strain name, country of origin, and year of detection are shown for each strain. GenBank accession numbers are presented in parentheses. The scale bar shows the genetic distance.