Table 1. Information on EV-D68-positive patients and EV-D68 detected in 2010–2015 in Osaka City, Japan.
| No. | Name | Specimens | Age | Gender | Diagnosis | Sampling Date | Co-detection | Clade | Sequenced Region | Nucleotides sequenced | GenBank No. |
|---|---|---|---|---|---|---|---|---|---|---|---|
| 1 | 705-OsakaC-JPN-2013 | Tracheal aspirate | 0 y 4 m | M | Viral bronchitis, respiratory failure | 2013/9/30 | Rhinovirus | A | Complete genome | 1–7341 | LC068709 |
| 2 | 726-OsakaC-JPN-2013 | Nasal mucus | 2 y 0 m | M | Asthmatic bronchitis | 2013/10/8 | Adenovirus | A | Complete genome | 1–7341 | LC068710 |
| 3 | 727-OsakaC-JPN-2013 | Nasal mucus | 6 y 0 m | F | Pneumonia | 2013/10/4 | A | Nearly complete genome | 48–7342 | LC068711 | |
| 4 | 752-OsakaC-JPN-2013 | Throat swab | 9 y 1 m | M | Asthmatic bronchitis | 2013/10/22 | ND* | VP4 | NR** | NR** | |
| 5 | 1082-OsakaC-JPN-2014 | Nasal mucus | 1 y 7 m | M | Pneumonia | 2014/12/1 | ND* | VP4 | NR** | NR** | |
| 6 | 594-OsakaC-JPN-2015 | Nasal mucus | 4 y 1 m | M | Asthma, bronchitis | 2015/8/15 | B (Subclade B3) | Nearly complete genome | 1–7296 | LC107895 | |
| 7 | 639-OsakaC-JPN-2015 | Nasal mucus | 3 y 10 m | M | Asthma, bronchitis | 2015/8/28 | B (Subclade B3) | Nearly complete genome | 1–7286 | LC107896 | |
| 8 | 676-OsakaC-JPN-2015 | Nasal mucus | 0 y 9 m | M | Asthmatic bronchitis | 2015/9/11 | ND* | Partial VP1 | NR** | NR** | |
| 9 | 712-OsakaC-JPN-2015 | Nasal mucus | 1 y 6 m | F | Pneumonia | 2015/9/20 | ND* | Partial VP1 | NR** | NR** | |
| 10 | 744-OsakaC-JPN-2015 | Nasal mucus | 1 y 7 m | F | Asthmatic bronchitis | 2015/10/5 | B (Subclade B3) | Complete VP1 | 927 | LC107890 | |
| 11 | 759-OsakaC-JPN-2015 | Nasal mucus | 3 y 8 m | F | Asthmatic bronchitis | 2015/10/8 | B (Subclade B3) | Complete VP1 | 927 | LC107891 | |
| 12 | A184-OsakaC-JPN-2015 | Throat swab | 0 y 2 m | F | Pertussis or viral ARI (suspected) | 2015/7/27 | B (Subclade B3) | Nearly complete genome | 1–7286 | LC107897 | |
| 13 | A194-OsakaC-JPN-2015 | Throat swab | 3 y 11 m | F | Pneumonia, athma | 2015/8/4 | B (Subclade B3) | Complete VP1 | 927 | LC107892 | |
| 14 | A216-OsakaC-JPN-2015 | Throat swab | 4 y 11 m | F | Bronchial asthma | 2015/8/18 | B (Subclade B3) | Complete VP1 | 927 | LC107893 | |
| 15 | A244-OsakaC-JPN-2015 | Nasopharyngeal aspirate | 0 y 7 m | F | Pneumonia | 2015/9/11 | Adenovirus | B (Subclade B3) | Nearly complete genome | 1–7298 | LC107899 |
| 16 | A250-OsakaC-JPN-2015 | Nasopharyngeal aspirate | 1 y 2 m | F | Pneumonia, athma | 2015/9/17 | B (Subclade B3) | Nearly complete genome | 1–7112 | LC107900 | |
| 17 | A252-OsakaC-JPN-2015 | Nasopharyngeal aspirate | 0 y 10 m | M | Bronchitis | 2015/9/18 | B (Subclade B3) | Nearly complete genome | 1–7110 | LC107901 | |
| 18 | A274-OsakaC-JPN-2015 | Throat swab | 0 y 10 m | M | Bronchial asthma | 2015/10/19 | B (Subclade B3) | Complete VP1 | 927 | LC107894 | |
| 19 | 200-OsakaC-JPN-2010 | Nasal mucus | 4 y | M | Bronchitis | 2010/6/4 | C | Complete VP1 | 927 | AB601872 | |
| 20 | 290-OsakaC-JPN-2010 | Nasal mucus | 0 y 7 m | F | Asthmatic bronchitis | 2010/6/26 | C | Complete genome | 1–7332 | AB601882 | |
| 21 | 373-OsakaC-JPN-2010 | Nasal mucus | 3 y 6 m | M | Bronchitis | 2010/7/17 | C | Complete VP1 | 927 | AB601873 | |
| 22 | 378-OsakaC-JPN-2010 | Nasal mucus | 1 y 6 m | F | Pneumonia | 2010/7/16 | C | Complete genome | 1–7331 | AB601883 | |
| 23 | 396-OsakaC-JPN-2010 | Nasal mucus | 4 y 6 m | F | Pneumonia | 2010/7/22 | C | Nearly complete genome | 1–6764 | AB601884 | |
| 24 | 402-OsakaC-JPN-2010 | Nasal mucus | 5 y 0 m | F | Asthma, respiratory failure | 2010/7/23 | C | Complete VP1 | 927 | AB601874 | |
| 25 | 412-OsakaC-JPN-2010 | Sputum | 3 y 9 m | M | Lower respiratory tract infection | 2010/7/23 | C | Complete VP1 | 927 | AB601875 | |
| 26 | 441-OsakaC-JPN-2010 | Nasal mucus | 1 y 3 m | F | Asthmatic bronchitis | 2010/8/1 | C | Complete VP1 | 927 | AB601876 | |
| 27 | 445-OsakaC-JPN-2010 | Sputum | 4 y 1 m | M | Asthmatic bronchitis | 2010/8/1 | C | Complete VP1 | 927 | AB601877 | |
| 28 | 471-OsakaC-JPN-2010 | Nasal mucus | 1 y 6 m | M | Bronchial pneumonia | 2010/8/5 | C | Complete VP1 | 927 | AB601878 | |
| 29 | 515-OsakaC-JPN-2010 | Throat swab | 0 y 3 m | M | Upper respiratory tract infection | 2010/8/19 | C | Partial VP1 | NR** | NR** | |
| 30 | 573-OsakaC-JPN-2010 | Throat swab | 3 y 5 m | M | Asthmatic bronchitis | 2010/9/1 | C | Complete VP1 | 927 | AB601879 | |
| 31 | 616-OsakaC-JPN-2010 | Nasal mucus | 0 y 7 m | M | Asthmatic bronchitis | 2010/9/13 | Adenovirus | C | Complete VP1 | 927 | AB601880 |
| 32 | 618-OsakaC-JPN-2010 | Nasal mucus | 1 y 5 m | M | Pneumonia | 2010/9/11 | C | Complete VP1 | 927 | AB601881 | |
| 33 | 404-OsakaC-JPN-2010 | Nasal mucus | 1 y 8 m | M | Febrile convulsion | 2010/7/25 | C | Nearly complete genome | 1–6764 | AB601885 | |
| 34 | 692-OsakaC-JPN-2013 | Nasal mucus | 0 y 9 m | M | Acute flaccid paralysis, diarrhea | 2013/9/24 | Human parechovirus 1 | A | Complete genome | 1–7341 | LC068708 |
| 35 | 685-OsakaC-JPN-2015 | Throat swab | 2 y 2 m | M | Unknown fever | 2015/9/15 | B (Subclade B3) | Complete VP1 | 927 | LC107889 | |
| 36 | A166-OsakaC-JPN-2015 | Throat swab | 1 y 10 m | F | Rash | 2015/7/10 | Human coronavirus OC43 | ND* | Partial VP1 | NR** | NR** |
| 37 | A241-OsakaC-JPN-2015 | Throat swab | 0 y 2 m | F | Fever | 2015/9/7 | B (Subclade B3) | Nearly complete genome | 1–7196 | LC107898 |
ND*: Not determined
NR**: Not registered
ARI: Acute respiratory infection
No. 1–18: EV-D68 positives with respiratory symptoms in this study.
No. 19–32: EV-D68 positives with respiratory symptoms in our previous study (15).
No. 33–37: EV-D68 positives without respiratory symptoms.