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. 2017 Aug 31;13(8):e1006587. doi: 10.1371/journal.ppat.1006587

Fig 8. Subcellular localization and colocalization of deletion mutants of Rep.

Fig 8

(A) Rep1-120-DsRed was expressed in epidermal cells of N. benthamiana leaves under CaMV 35S promoter. At 3 dpi, all observed cells (n = 15) showed fluorescence of Rep1-120-DsRed in the nucleus and in the nucleolus (arrow). (B) Rep1-180-DsRed fluorescence was restricted throughout the nucleus except the nucleolus (arrow). (C) Colocalization of NbHUB1-GFP and Rep1-120-DsRed was observed. (D) Rep1-180-DsRed was colocalized in the nucleus with NbHUB1-GFP. (E) Rep1-120-DsRed colocalized with NbUBC2-EGFP in nucleus. (F) Rep1-180-DsRed also colocalized with NbUBC2-EGFP in nucleus. Scale bars = 20 μm.