Table 1.

Comparison of TaqMan assay-based real-time quantitative PCR and RNA-seq analyses of 8 genes differentially expressed between 2D and 3D cells. The TaqMan P is the t test p value from TaqMan data analysis for each gene. The log base 2-fold change (log2FC) correlation coefficient between TaqMan and RNA-seq is 0.997 (P = 3.467E-8).

Gene symbol	RNAseq_mean log2FC	TaqMan_mean log2FC	TaqMan P (Bonferroni)
ATP5O	−1.02	−0.83	1.14E-02
BDH2	−2.28	−2.80	2.88E-03
COL5A2	−3.58	−4.06	4.12E-06
DEPDC1	−5.09	−5.09	7.72E-05
FKBP8	−1.02	−1.49	3.51E-04
IL6	7.55	7.18	1.73E-05
NFIL3	2.77	2.73	4.03E-05
TFRC	1.60	1.63	5.29E-05