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. 2017 Apr 13;8(4):353–359. doi: 10.1080/19491034.2017.1313937

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© 2017 The Author(s). Published with license by Taylor & Francis.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 3. — (A) A simple scheme for oblique illumination microscopy. An illumination laser (green) and focal plane (red) in a living cell are shown. (B) Single-nucleosome image of a human HeLa cell nucleus expressing H2B-PA-mCherry. Each dot represents a single nucleosome (adopted from ref. 24). (C) Representative trajectories of fluorescently labeled single nucleosomes (50 ms per time frame). (D) Plots of the MSD at the interior (orange) and periphery (green) regions. Each plot fits well with the MSD curve for subdiffusion (Eq. (1)).