Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Sep 15;28(19):2492–2507. doi: 10.1091/mbc.E17-01-0022

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2017 Mathiowetz, Baple, et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

“ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology.

PMC Copyright notice

FIGURE 2: — Truncated WHAMM proteins exhibit altered properties in cells. (A) Fibroblasts from two healthy (+/+) Amish individuals or Amish GMS (GMS/GMS) patients were fixed and stained with anti-CC antibodies to visualize WHAMM (green). Nuclei, based on DAPI staining, were denoted with dashed ovals. (B) Cos7 cells were transfected with plasmids encoding a vector control, HA-WHAMM(WT), (Δ7), or (X6) and blotted with anti-HA or anti-tubulin antibodies. (C) Cos7 cells transiently expressing LAP-WHAMM(WT), (Δ7), or (X6) (green due to the GFP tag) were fixed and stained with phalloidin to visualize F-actin (red) and with DAPI to label DNA (blue). (D) NIH3T3 cells stably transfected with LAP-WHAMM(WT) or (Δ7) or a LAP vector control were fixed and stained as in C. All scale bars: 10 μm.