Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Sep 15;28(19):2492–2507. doi: 10.1091/mbc.E17-01-0022

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2017 Mathiowetz, Baple, et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

“ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology.

PMC Copyright notice

FIGURE 3: — Amish GMS WHAMM truncations do not promote actin nucleation but can associate with microtubules. (A) Purified MBP-WHAMM(WT), (Δ7), and (X6) were analyzed by SDS–PAGE and Coomassie blue staining or anti-MBP immunoblotting. WHAMM is abbreviated WHM. (B) Actin was polymerized in the presence of the Arp2/3 complex and either MBP, MBP-WHAMM(WT), (Δ7), or (X6). The amount of F-actin, in arbitrary fluorescent units (AU), formed over time is shown. (C) Microtubules were incubated in the presence of MBP-WHAMM(WT) or (Δ7), and collected by centrifugation. Proteins found in pellet (P) and supernatant (S) samples were separated by SDS–PAGE. Tubulin was visualized by Ponceau S staining, while WHAMM fusions were detected by anti-MBP blotting. (D) MBP-WHAMM(X6) was incubated in the presence or absence of microtubules and processed as in C. (E) Cos7 cells transiently coexpressing LAP-WHAMM(WT) and mCherry-WHAMM(X6) were fixed and stained with antibodies to visualize microtubules.