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. 2017 Sep 15;28(19):2492–2507. doi: 10.1091/mbc.E17-01-0022

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© 2017 Mathiowetz, Baple, et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 8: — WHAMM localizes to omegasomes and subdomains of nascent autophagosomes. (A) Cos7 cells transiently expressing mCherry-WHAMM (red) and GFP-DFCP1 (green) were starved and stained with DAPI to label DNA (blue). Arrows indicate areas of WHAMM and DFCP1 colocalization; arrowheads highlight a region of WHAMM and DFCP1 juxtaposition. Scale bars: 10 μm. (B) Cos7 cells transiently expressing mCherry-WHAMM (red) and GFP-DFCP1 (green) were starved and stained with phalloidin to label F-actin (pink). Scale bars: 1.5 μm. (C) Cos7 cells transiently expressing GFP-WHAMM or GFP-WDR73 (green) were starved and stained with antibodies to label LC3 (red). Individual autophagosomal structures with punctate GFP-WHAMM localization to regions of membrane growth or curvature are highlighted in this composite. Scale bars: 1.5 μm.