TABLE 2:
Oligonucleotides used in this study.
| Primer name | Sequence (5′→3′) |
|---|---|
| Stv1-50F2 | GGCCTTCCAATCGATTGTGGATGCATACGGTATCGCAACATATAAAGAAATCAATCGGATCCCCGGGTTAATTAA |
| Stv1-50R1 | GGACATTGCCTCCACCCAATGCAAACGTAGCGCATGCAACATTGCGGAAGGAATTCGAGCTCGTTTAAAC |
| Vph1FL-F2 | GAGTATAAAGACATGGAAGTCGCTGTTGCTAGTGCAAGCTCTTCCGCTTCAAGCCGGATCCCCGGGTTAATTAA |
| Vph1FL-R1 | CTCACTATATAGTAGCATCATTTATTATTTAATGAAGTACTTAAATGTTTCGCGAATTCGAGCTCGTTTAAAC |
| STV1-URA-UP | CCAGTTGAGGCGTTTCGATGAAGTGGAAAGGATGGTAGGCTTCTTGAATGAGGTACTGAGAGTGCACCACGCT |
| STV1-URA-DOWN | GGATCAATTCTGTGGAAGGCACCCAACCTTCGGCTATTAGACCCTGTGATCAGTTTTTTAGTTTTGCTGGCC |
| STV1 2391ORF | GGCACTATCGTTGGCGCATG |
| STV1+500 | TACAGCAGAGATTTATGGTATGCC |
| Vph1-1 | GAGGTATTTAGAAGTGAAGAGAC |
| Vph1-C4 | AACGTTTCATGAGATAAGTTTGGC |
| Stv1NT-FLAG-1 | GCGGATCCATGAATCAAGAAGAGGCTATATTC |
| Stv1NT-FLAG-2 | GCGTGCAGTTACTTGTCATCGTCATCCTTATAGTCTGTTGCGATACGCTATGCATC |
| Stv1-418a | GTCATCGTCATCCTTATAGTCTAGGATCACATTAAAGACGGT |
| Stv1-418b | ACCGTCTTTAATGTGATCCTAGACTATAAGGATGACGATGAC |
| a2_1-364(KpnI)F | TTAGCCGGTACCAATGGGGTCCCTGTTCCG |
| a2_1-364(HindIII)R | TTACCAAAGCTTCTATTCTTTTGTGGGGATTATATTCATGAATGAGGGGATTG |
| Hua2-FLAG-For | AAGGACGACGATGACAAGTAGAAGCTTGGCACTGGCCGTCGT |
| Hua2-FLAG-Rev | GTCATCGTCGTCCTTGTAATCTTCTTTTGTGGGGATTATATTCATG |