Figure 3. Effects of septate junction components overexpression on blood cell homeostasis.
(a–c’) Validation of myc-tagged Cora or NrxIV over-expression using collier-Gal4 mediated PSC- specific over-expression using anti-myc tag antibody as compared to its control. P1 positive plasmatocyte counts (d’) remain unaltered upon collier-Gal4 mediated over-expression of Cora whereas they are completely suppressed upon collier-Gal4 mediated over-expression of NrxIV (d’’) as compared to their respective controls (d). Hnt positive crystal cell counts are not affected upon collier-Gal4 mediated over-expression of Cora (e’) or Nrx (e’’) as compared to control (e). (f–f’) Quantitation of plasmatocyte and crystal cell counts upon collier-Gal4 mediated over-expression of cora or nrxIV. PSC is co-labeled in green with collier-Gal4 driven GFP. Plasmatocytes are labeled with P1 (Red), crystal cells with Hnt (Red). Nuclei are labeled with DAPI (Blue). ns indicates non-significant. Error bars represent s.d. Scale Bar: (a–c’) 40 μm, (d–e’’) 50 μm.
Figure 3—figure supplement 1. PSC- specific over-expression of Cora or NrxIV does not alter the PSC size.
