Fig. 3.

TGFB1 reduces DNA synthesis in luteal fibroblasts. A) [³H]thymidine incorporation assay of fibroblasts plated at low density and treated with or without TGFB1 (0–10 ng/ml) for 24 h. Some cells were cultured in the presence of 5% FCS alone or in combination with TGFB1 (1 ng/ml) for 24 h. Data are expressed as the percentage incorporation of [³H]thymidine compared to the maximal response group (5% FCS). Data represent three independent experiments, each performed in triplicate (mean ± SEM, n = 3, *P < 0.05). B) [³H]thymidine incorporation assay of fibroblasts plated at low density and treated for 24 h with or without TGFB1 (1 ng/ml) in the absence (Ctl) or presence of FGF2 (10 ng/ml) and 5% FCS. Data are expressed as the percentage incorporation of [³H]thymidine compared to the maximal response group (5% FCS). Data represent three independent experiments, each performed in triplicate (mean ± SEM, n = 3, *P < 0.05).