Fig. 4.

TGFB1 does not reduce viability of luteal fibroblasts. A) MTT assay of fibroblasts plated on plastic at high density and treated for 24 and 48 h without or with TGFB1 (0–10 ng/ml) in the absence of FCS. Data represent three independent experiments, each performed in triplicate (mean ± SEM, n = 3, P > 0.05 vs. 0 ng/ml of TGFB1). B) MTT assay of fibroblasts plated on plastic at high density and treated for 48 h with control medium (Control) or TGFB1 (1 ng/ml), alone or in combination with control medium (Ctl), TNF (50 ng/ml), IFNG (50 ng/ml), or FASLG (100 ng/ml) in the absence of FCS. Results are expressed as the percentage absorbance observed in the control group. Data shown represent three independent experiments, each performed in triplicate (mean ± SEM, n = 3, P > 0.05 vs. Ctl).