Figure 4. Lfng expression, somite patterning, and skeletal morphology are normal in mir-125a-5p mutant mice.

A) Skeletal morphology was assessed by alizarin red /alcian blue staining. Normal skeletal morphology and somite patterning are observed in wild type (a), mir-125a-5p^Δ11 (b) and mir-125a-5p^Δ3T (c) embryos. B). Lfng expression at 10.5 d.p.c. is observed in all three phases of oscillatory expression in wild type, mir-125a-5p^Δ11 and mir-125a-5p^Δ3T embryos. C) Expression profiles of multiple embryos further support the idea that Lfng expression patterns are unperturbed after the loss of mir-125a-5p. D) Somite patterning was examined using the caudal marker Uncx. Normal somite patterning is observed in wild type (a), mir-125a-5p^Δ11 (b) and mir-125a-5p^Δ3T (c) embryos. Quantification of somites, vertebrae, and ribs (data not shown) confirms normal somitogenesis and skeletal development in the absence of mir-125a-5p.