Figure 2. RC-101 inhibits TLR4-dependent signaling in mouse Mϕs.

Peritoneal Mϕs were treated with the indicated dose of RC-101 or mock control (PBS) immediately before treatment with LPS (100 ng/ml). Peritoneal Mϕ lysates were harvested at the indicated times, separated by denaturing SDS-PAGE, and probed with Abs directed against the following phosphorylated proteins and loading controls: TBK1, NF-κB p65, ERK1/2, JNK, p38. The housekeeping protein β-tubulin and unphosphorylated proteins were used as loading controls. A representative blot from 3 independent experiments is shown.