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. 2017 Jul 20;102(4):1103–1113. doi: 10.1189/jlb.2A1215-567RR

Figure 4. RC-101 fails to destabilize the ligand-independent TLR4 oligomerization in HEK293T cells.

Figure 4.

(A and B) Mouse Mϕs were stimulated with increasing doses of LPS for 60 min and analyzed for expression of IL-1β (A) and TNF-α (B). The arrow represents the dose of LPS used throughout the study. The data represent the means ± sd for experimental conditions performed in duplicate. A representative of 4 independent experiments is shown. (C) HEK293T cells were transfected with the indicated expression plasmids and incubated for 48 h. Whole-cell lysates were separated by SDS-PAGE and blotted for transgene expression (input) or immunoprecipitated with anti-FLAG–specific Ab. Immunoprecipitated samples were separated by SDS-PAGE and probed with anti-FLAG or anti-CFP specific Abs. (D) Same as (C), except RC-101 (30 μg/ml) was added where noted 30 min before harvesting of the whole-cell lysates. For (C) and (D), representative blots from 1 of 3 independent immunoprecipitation reactions are shown.