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. 2017 Jun 29;102(4):953–964. doi: 10.1189/jlb.6HI0217-043R

Figure 1. Monocyte PSGL-1 and plasma glutamate are induced with HIV infection and murine EcoHIV infection.

Figure 1.

(A) Whole blood was collected from 32 HIV− donors and 28 cART-treated HIV+ donors with undetectable viral loads and analyzed by flow cytometry. Monocytes were identified by FSC/SSC and then CD14 staining and analyzed for PSGL-1 MFI. PSGL-1 is increased significantly with HIV infection. ***P = 0.0204, Student’s t-test. (B) Monocytes subsets were identified by CD14 and CD16 staining, and (C) PSGL-1 expression was determined in each subset and analyzed by 2-way ANOVA F-test. HIV+ vs. HIV−: P = 0.0011; subsets: P = 0.0068; post hoc tests: HIV+ vs. HIV−; Classic: **P < 0.01; Intermediate: **P < 0.01. (D) WT C57BL/6 mice (n = 6) and CD40 KO (n = 6) mice were infected with EcoHIV (1.0 × 105 pg p24), and blood was collected by cardiac exsanguination at 2 wk postinfection and analyzed by flow cytometry. Monocytes were identified by FSC/SSC and then by CD115 and Gr-1 staining and analyzed for PSGL-1 expression. Monocyte PSGL-1 expression is increased with EcoHIV infection in a CD40L-dependent manner. Two-way ANOVA, interaction: P = 0.0269; post hoc test: Uninfected vs. EcoHIV Infected, *P < 0.05.