Figure 7. c-Myc-MAX dimerization inhibition by 10058-F4 prevents sCD40L + glutamate-induced PSGL-1 on monocytes ex vivo.

Monocytes were isolated from 3 HIV⁻ donors by positive selection against CD14 after density gradient isolation of PBMCs. The monocytes were treated with glutamate and sCD40L and/or the c-Myc/MAX dimerization inhibitor, 10058-F4, for 24 h, and PSGL-1 expression was determined by flow cytometry. c-Myc/MAX is required for sCD40L + glutamate-induced PSGL-1 expression on monocytes ex vivo Two-way ANOVA, Treatment: P = 0.0078, Inhibitor: P < 0.0001, Interaction: P = 0.0303, Post-test: not treated, without CD40L + glutamate (NT) vs. treated, with CD40L + glutamate (T), P < 0.01, **P < 0.01, ***P < 0.001. NI, No inhibitor, without 10058-F4.