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. 2017 Jul 28;102(4):1143–1151. doi: 10.1189/jlb.5A1016-445R

Figure 4. UPR markers in HL60 models.

Figure 4.

(A) HL60 cells were transfected (electroporation) with WT and mutant (P139L, G214R, C151Y, V174_C181del) NE-expressing vectors, cultured for 48 h, lysed, and Western blots were performed using anti-BiP and anti-ATF6 Abs as described in Materials and methods. (B) Tet-regulated HL60 cells expressing WT or mutant NE were cultured for 72 h in the presence or absence of doxycycline, lysed, and Western blots were performed as described. Representative experiments are shown (n = 3).