Figure 5. LRRK2 G2019S gene in myeloid cells suppresses Th17 cell differentiation in vitro.

(A) Effects of in vitro–cultured myeloid cells on CD4⁺ T cell differentiation. LPS-activated myeloid cells were cocultured with rat spleen CD4⁺ T cells in the presence of SEB (5 μg/ml) for 5–6 d. Frequencies of IL-17⁺ Th17 cells and IFN-γ⁺ Th1 cells were examined after coculture. (B) Expression of selected genes in cultured myeloid cells. Rat bone marrow cells were differentiated with M-CSF (20 ng/ml) for 7 d and then activated with LPS for 24 h before quantitative RT-PCR analysis of indicated genes. Representative dot plots and pooled data (n = 5) are shown. *Significant differences (P ≤ 0.05) from control groups. Error bars indicate means ± sem.