Figure 5. Pyk2 regulates Ly6C⁻ BM monocyte survival in vivo.

(A) Representative contour plots display Annexin V staining and DAPI incorporation in BM Ly6C⁻ monocytes from WT and Pyk2^−/− mice, assessed by flow cytometry. The adjacent graphs show the average percentage of apoptotic (DAPI⁻/Annexin V⁺, left) or dead (DAPI⁺/Annexin V⁺, right) monocytes in BM monocyte subpopulations (WT, n = 8; Pyk2^−/−, n = 8). Data were acquired from 2 independent experiments. (B) Representative contour plots display active caspase (FLICA) staining in BM Ly6C⁻ monocytes from WT and Pyk2^−/− mice, assessed by flow cytometry. The adjacent graph shows the percentage of BM monocyte subsets expressing cleaved (active) caspases, assessed by flow cytometry (WT, n = 8; Pyk2^−/−, n = 8). Data were acquired from 2 independent experiments. (C) Percentage of BM monocyte subsets staining positive for Annexin V (left), DAPI (middle), or cleaved caspases (right) in WT→WT and Pyk2^−/−→WT chimeric mice, assessed by flow cytometry (WT→WT, n = 12; Pyk2^−/−→WT, n = 12). (D) Quantification of the mean fluorescence intensity of cell-surface CD115 (M-CSFR) for WT and Pyk2^−/− BM monocyte subpopulations. *P < 0.05; **P < 0.01; ***P < 0.001 (unpaired Student’s t test).