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. 2017 Sep 13;7:11443. doi: 10.1038/s41598-017-11936-0

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Procedure for in planta particle bombardment (iPB) transformation of wheat. (a) Coleoptiles and leaf primordia from mature embryos were excised under a microscope, (b) and arranged on a culture plate with Murashige and Skoog’s (MS) medium before (c) transformation. (d) Bright field and (e) fluorescence-merged images of the whole apical tissues 12 h after bombardment. (f) Bright field (upper) and fluorescence (lower) images of a single apical tissue. (g) Close-up images of a SAM region of apical tissue. (h) Wheat plants grown on MS medium 3 weeks after bombardment. (i) Bombarded wheat plants 2 weeks after transfer to soil. Shoot apical meristems (SAMs) are indicated by arrows in panels (b), (f) and (g).