Figure 3.

Expression of GFP in transgenic wheat plants. (a) Reverse transcription polymerase chain reaction (RT-PCR) analysis of GFP in ‘Fielder’ lines (FG1–5) and wild-type (Wt) plants. The GAPDH gene (Genbank accession number: EF592180) was used as a housekeeping control. Full-length gel images are shown in Supplementary Fig. S7. (b) Immunoblot analysis of green fluorescent protein (GFP) in transgenic plants (FG1–5). Total protein (20 µg per lane) extracted from each T₁ leaf tissue was separated using sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) and blotted. GFP was detected with anti-GFP antibodies. The full-length gel image is shown in Supplementary Fig. S8. (c) GFP accumulation in T₁ seeds of a transgenic wheat line (FG1). Left: fluorescence image of whole T₁ seeds harvested from the primary panicle of FG1. Middle: close-up views of T₁ (upper) and Wt (lower) seeds. Right: fluorescence images of sections of FG1 T₁ (upper) and Wt (lower) seeds. The aleurone cell layer is indicated by an arrow. (d) Bright-field (BF, left) and GFP (right) images of T₁ seedlings in Wt (top) and FG1 (bottom) lines.