Figure 4.

Integration and expression of the GFP gene in transgenic ‘Haruyokoi’. (a) Genomic polymerase chain reaction (PCR) analysis of four T₁ transgenic (HG1–4) lines and wild-type (Wt) plants. Genomic DNA was extracted from each first leaf. The full-length gel image is shown in Supplementary Fig. S5. (b) Reverse transcription-PCR (RT-PCR) analysis of GFP expression in HG1–4 lines and Wt plants. The GAPDH gene was used as a housekeeping control. Full-length gel images are shown in Supplementary Fig. S6. (c) DNA gel blot analysis of transgenic wheat lines (T₂ progeny) and Wt lines using genomic DNA from leaves digested with HindIII. Two-hundred picograms of the linearised GFP vector (5.1 kb) used as a positive control (P).