Figure 1.

Life history parameters for Psyttalia concolor reared on either wild-type or OX3097D-Bol Bactrocera oleae larvae. Wild-type (n = 720) and OX3097D-Bol (n = 720) B. oleae (olive fly) third instar larvae reared in the presence of 100 μg/mL of tetracycline were presented to P. concolor naïve females (n = 240) at a 1:3 ratio (parasitoid female: olive fly larvae) for 30 min. Approximately 20% of the parasitized olive fly larvae (n = 144) were dissected to determine the level of parasitism. The remaining 80% of parasitized olive fly larvae (n = 576) were transferred to Petri dishes of fresh diet without tetracycline and allowed to emerge. (A) Percentage emergence of adult olive fly parasitized by P. concolor. Emergence from parasitized olive fly was estimated to be 0.35% and 0.17% for wild-type and OX3097D-Bol, respectively. Emergence from non-parasitized control olive fly was 73.3% for wild-type and 62.5% for OX3097D-Bol. (B) Mean number of P. concolor eggs laid into olive fly larvae. (C) Percentage of emergence of adult P. concolor from the parasitized olive fly larvae. (D) Mean number of days from parasitism to the emergence of adult male wasps (developmental days). (E) Mean number of days from parasitism to the emergence of adult female wasps (developmental days). (F) Mean number of eggs in P. concolor female ovaries. For non-proportional data, the numbers represent mean and standard error of the mean (±SEM). P-values ≤0.05 were considered statistically significant.